Dr Kerry Gilmore
Research Fellow, University of Wollongong
PhD (Cell Biology) UOW
Summary of Current Research:
My current research at IPRI, within ACES, involves the utilization of conducting organic materials for the electrical stimulation of electrically responsive cells including nerve and muscle. Electrical stimuli applied directly to attached neural cell lines and primary neural tissue via conducting polymers including polypyrroles and polythiophenes have been shown by researchers within ACES to enhance the differentiation of these cell types. Conducting polymers have also been shown to provide supportive substrates for the proliferation and differentiation of skeletal muscle primary myoblasts and cell lines, and stem cells including neural progenitor cells and adipose stem cells. My current research projects aim to expand these studies by applying direct and/or field electrical stimulation to nerve, adipose stem and primary skeletal muscle cells supported within 3D environments, which have been shown to mimic more closely the in vivo cell millieu.
In conjunction with the team at ACES we will utilize the state-of-the-art printing facilities to design and print 3D electrochemical cells and cultured cell printing systems using a selection of the broad range of conducting and hydrogel systems that have been developed in Wollongong. The effects of electrical stimulation on cells in 3D environments will be assessed using the expanded range of techniques now available at ACES including cell surface marker detection via Flow Cytometry, Immunostaining and Confocal Microscopy, gene expression studies using RT and Real Time PCR, protein expression studies using SDS-PAGE and Western Blotting as well as biochemical assays and DNA quantitation for measurement of cell proliferation.
My interest in the process of cell death (apoptosis) was initiated during my PhD studies at the University of Wollongong. The project was an exploration of the molecular mechanisms of apoptosis utilising techniques such as Flow Cytometry, immunohistochemistry and imaging of cells using Confocal Microscopy and analysis of protein expression using SDS-PAGE and Western Blotting.
The study of apoptosis continued with post-doctoral research completed at the University of Notre Dame in South Bend, Indiana, USA on the mechanisms of action of the breast cancer drug, tamoxifen. This study utilized techniques including cell proliferation assays such as MTT, breast cancer cell invasion assays, protein expression to characterize siRNA knockdown of oestrogen receptors using SDS-PAGE and Western Blotting and Flow Cytometry.
ASBTE (Australian Society for Biomaterials and Tissue Engineering)
Interested in the ACES Bionics program? Contact Dr Gilmore
phone: +61 (02) 4298 1437